Biology Lab Report Essay Example | Topics and Well Written Essays - 750 words

Biology Lab Report - Essay Example Quaternary Disinfectant Cleaner (concentrate), 2007). It may come in concentrated pack indicated as: Pseudomonacidal, Staphylocidal, Salmonellacidal, Bactericidal, Fungicidal, Virucidal, and mildewstatic disinfectant (LYSOL Brand I.C. Quaternary Disinfectant Cleaner (concentrate), 2007). It is also commonly known as decontaminant in almost all spheres where microorganisms are not welcome (LYSOL Brand I.C. Quaternary Disinfectant Cleaner (concentrate), 2007). Its active ingredients are Didecyl dimethyl ammonium chloride, 10.14%, and n-Alykl (C14 50%, C12 40%, C16 10%) dimethyl benzyl ammonium chloride , 6.76%. Its inert ingredients are 83.10% (LYSOL Brand I.C. Quaternary Disinfectant Cleaner (concentrate), 2007. According to Reckitt Benckiser the company of professionals that market Lysol, the product has been found to be effective at controlling transmission by eradication of common microorganisms that have been the etiologic agents of human and animal diseases such as colds diarrhea, Rhinovirus (LYSOL Brand I.C. Quaternary Disinfectant Cleaner (concentrate), 2007). Thus, Lysol is anti-bacterial, anti-fungal, and anti-virus agent (LYSOL Brand I.C. Quaternary Disinfectant Cleaner (concentrate), 2007). However, its capability of inhibiting growth of Serratia marcescens must be tested. Serratia marcescens is the common strain of bacteria normally used as laboratory experimental subjects. This bacterial strain has a characteristic colony color of red, and has been found to be selectively pathogenic to human beings (Owens & McGowan, 1999). As pathogenic organisms, they are capable of causing infectious diseases (Owens & McGowan, 1999). They may also be contaminants of materials (Owens & McGowan, 1999). However, they can be easily eradicated by physical contact with Lysol as proven by the professionals of Reckitt Benckiser company (LYSOL Brand I.C. Quaternary Disinfectant Cleaner (concentrate), 2007). Statement of the question Does Lysol inhibit the growth of Serratia marcescens HypothesisHo: Lysol does not inhibit growth of Serratia marcescens. Description of Experiment The control set-up consist of 2 Petri dishes of agar streaked with Serratia marcescens marked O% Lysol, and the experimental Set-up consist of 10 Petri dishes of agar streaked with Serratia marcescens marked 100%, 50%, 25%, 12.5%, and 6.25% Lysol. In addition, 12 small paper dots, and 50 ml per concentration of Lysol was prepared. PredictionIf a solution contains high concentration of Lysol, then there will be greater zone of inhibition on a plate streaked with Serratia marcescens, and lower concentrations will not show, if at all, zones of inhibition. Significance of Prediction The extent of zones of inhibitions would reveal Lysol's effects on Serratia marcescens, whether at high concentration or at low concentration. Methods and Materials a) Independent variable i) Lysol, Serratia marcescens, and 1 week were the independent variables. ii) Lysol was prepared at: 100%, 50%, 25%, 12.5%, and 6.25% concentrations, while Serratia marcescens was streak on 12 agar plates. iii) These were chosen to determine at what concentration of Lysol will there be greater or lower zones of inhibitions on Serratia marcescens as streaked on 12 agar plates. iv) 0% Lysol was indicated for the control group. b) Dependent variables i) The zones